People

people

Muhammad Saleem, PhD

Postdoctoral Fellows

Research Interests

​Dr. Saleem is interested in eukaryotic and prokaryotic proteins expression, purification, characterization, and high-resolution structure determination by x-ray crystallography and cryo electron microscopy; Self-assembly of recombinant proteins and peptides for therapeutic applications

Selected Publications

Characterisation of the Immunomodulatory Effects of Meningococcal Opa Proteins on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
C. Jones, M. Sadarangani, S. Lewis, I. Payne, M. Saleem, J.P. Derrick and A. Pollard
PlosOne, 11(4) e0154153, 2016

Dissection of the function of the RmpM periplasmic protein from Neisseria meningitides
S. Maharjan, M. Saleem, I.M. Feavers, J.X. Wheeler, R. Care, J.P. Derrick
Microbiology, 162(2): 364-375, 2016

PqsBC, a condensing enzyme in the biosynthesis of the Pseudomonas aeruginosa quinolone signal: crystal structure, inhibition, and reaction mechanism
S.L. Drees, C. Li, F. Prasetya, M. Saleem, I. Dreveny, P. Williams, U. Hennecke, J. Emsley, and S. Fetzner
Journal of Biological Chemistry 291(13): 6610-6624, 2016

Structures of NCS-1 complexes with the D2 dopamine receptor and GPCR kinase 1 (GRK1) peptides shows different modes of interactions
S. Pandalaneni, V. Karuppiah, M. Saleem, L.P. Haynes, R.D. Burgoyne, O. Mayans, J.P. Derrick, and L.Y. Lian
Journal of Biological Chemistry, 290(30): 18744-18756, 2015

N. meningitidis native outer membrane vesicles containing modified LPS have an adjuvant effect for meningococcal, non-meningococcal proteins, and polysaccharide antigens
J.C. Nagaputra, C. Rollier, M. Sadarangani, J.C. Hoe, O. Mehta, G. Norheim, M. Saleem, J.P. Derrick, H. Chan I. Feavers, A. Pollard, E.R. Moxon
Clinical and Vaccine immunology, 21(2): 234-242, 2014

Use of a molecular decoy to segregate transport from antigenicity in the FrpB iron transporter from Neisseria meningitides
M. Saleem, S.M. Prince, S. Rigby, M. Imran, H. Sanders, H. Patel, H. Chan, I.M. Feavers and J.P. Derrick
PlosOne, 8(2) e56746, 2013


Refolding, purification and crystallization of the FrpB outer membrane iron transporter from Neisseria meningitides
M. Saleem, S.M. Prince, H. Patel, H. Chan, I.M. Feavers and J.P. Derrick
Acta Crystallographica, F68: 231-235, 2012

Expression, purification, and crystallization of Neisserial outer membrane proteins
M. Saleem, J. Moore and J.P.  Derrick
Methods in Molecular Biology. 799: 91-106, 2012

Purification and 3-D electron microscopy structure of the Neisseria meningitides type IV pilus biogenesis protein PilG
R.F. Collins, M. Saleem and J.P. Derrick
Journal of Bacteriology 189: 6389-6396 2007

Education

  • ​Ph.D., Biomolecular sciences, The University of Manchester, U.K., 2008
  • M.Phil., Biotechnology, National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan, 2004
  • M.Sc., Chemistry, University of the Punjab, Pakistan, 2002
  • B.Sc., Chemistry, Zoology University of the Punjab, Pakistan, 1999

Professional Profile

  • ​2017 –Present: Postdoctoral Fellow, King Abdullah University of Science and Technology (KAUST), Saudi Arabia
  • 2016 – 2017: Postdoctoral Research Fellow, University of Nottingham, U.K.
  • 2013 – 2015: Postdoctoral Research Fellow, University of Leeds, U.K.
  • 2008 – 2013: Postdoctoral Research Fellow, University of Manchester, U.K

Scientific and Professional Memberships

  • ​British crystallographic association, BCA
  • Society of general microbiology, SG

Awards

  • ​2005-2008 BBSRC and The University of Manchester UK scholarship for PhD