A chemically well-defined, self-assembling 3D substrate for long-term culture of human pluripotent stem cells
Y. Loo, Y.S. Chan, I. Szczerbinska, B.C.P. Tan, A.C.A. Wan, H.H. Ng, C.A.E. Hauser
ACS Appl. Bio Mater., 2, 4, 1406-1412, 2019
Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation, and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol–gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
"KAUST shall be a beacon for peace, hope and reconciliation, and shall serve the people of the Kingdom and the world."
King Abdullah bin Abdulaziz Al Saud, 1924 – 2015
Thuwal 23955-6900, Kingdom of Saudi Arabia
Al-Haytham Building (Bldg. 2)